基于BTO多目标遗传算法的播种机性能优化设计

受播种地形和地域的影响,大部分播种机的播种质量、播种效率和能耗不能发挥到最佳状态,为了提高播种机的作业性能,深入挖掘了按顾客订单生产(Build-to-Order,BTO)模式的一般性意义,并将该模式引入到了播种机的优化设计中,提出了一种基于BTO模式的多目标速度控制遗传算法优化模型。根据播种质量、效率和能耗的要求,首先确定了BTO模式下播种机性能优化的初始参数,利用多目标函数确定了播种机的速度控制模型,并利用遗传算法进行了优化设计。最后,通过试验样机对播种机的播种性能进行了测试,结果表明:使用多目标遗传算法对播种机的性能进行优化后,播种机的播种质量、播种效率和播种能耗有了明显的改善,为新型播种机的研发提供了较有价值的参考。     

牛病毒性腹泻病毒E0-E2基因融合腺病毒的构建及小鼠免疫效果评价

【目的】 研究牛病毒性腹泻病毒（Bovine viral diarrhea virus,BVDV）E0和E2串联基因重组腺病毒作为基因工程疫苗的应用潜力。【方法】 采用PCR扩增、E0-E2基因融合并构建重组穿梭质粒pDC316-E0-E2,将其与AdMax腺病毒系统的骨架质粒共转染HEK293T细胞包装成重组腺病毒,通过Western blotting进行验证,并通过Reed-Muench法测定病毒滴度,通过肌内、皮下免疫接种小鼠后用ELISA方法及流式细胞检测进行免疫效果试验。【结果】 成功扩增到E0、E2基因目的片段,大小分别为681和1 122 bp,得到了完整的腺病毒Ad5-E0-E2;测定其滴度为1.1×10¹⁰ PFU/mL;Western blotting检测结果显示,Ad5-E0-E2外源基因在HEK293T细胞中表达,得到了与预期相符的目的条带（65 ku）;ELISA检测结果表明,通过肌内和皮下注射Ad5-E0-E2均能产生较高的抗体水平;流式细胞检测显示首免、二免后肌内和皮下注射Ad5-E0-E2组CD4^＋、CD4^＋/CD8^＋比值均极显著高于PBS对照组（P<0.01）。【结论】 本试验成功构建重组腺病毒Ad5-E0-E2,且具有较好的反应原性和免疫原性,能诱导机体产生针对BVDV的特异性抗体。     

Cell lines and plants obtained after protoplast fusions of Rosa+Rosa,Rosa+Prunus and Rosa+Rubus

Summary Protoplasts of three Rosa cultivars were fused with each other, with protoplasts of Prunus `Colt' and with protoplasts of Rubus laciniatus, using polyethylene glycol 4000 as a fusogen. Protoplasts of Prunus were incapable of cell division and those of Rosa and Rubus were disabled by treatments with metabolic inhibitors, either iodoacetate (IOA) or rhodamine 6-G (R6G). Parental protoplasts were then fused in combinations that required complementation for their survival. RAPD analysis of 41 fusion-derived cell lines showed that two lines resulting from fusions of Rosa + Rosa and one from a fusion of Rosa + Prunus, contained some DNA markers from both fusion partners. The others contained markers of only one fusion parent. This showed that after protoplast fusion, the heterokaryons did not develop into cell lines with stable hybrid nuclei. Plants regenerated from cell lines derived from Rosa + Prunus and Rosa + Rubus fusions contained DNA markers of only Rosa and their DNA amounts were no greater than that of the Rosa parent. However, they differed morphologically from the Rosa parent to a remarkable degree, possibly because they inherited undetected genes of Prunus or Rubus, or because they were somaclonal variants of the Rosa parent. Alternative strategies for the production of somatic hybrids are discussed.     

Further manipulation by centric misdivision of the 1RS.1BL translocation in wheat

Complete chromosomes 1R and 1B were reconstructed in wheat from the centric wheat-rye translocation 1RS.1BL. Three substitutions: 1R(1A), 1R(1B), 1R(1D), and three new centric translocations: 1RS.1AL, 1RS.1BL, 1RS.1DL were produced from the reconstructed chromosome 1R. Each one of these has the same rye chromosome arm 1RS which was present in the original translocation 1RS.1BL of ‘Kavkaz’ wheat. Reconstructed chromosome 1B and a normal chromosome 1R were used to produce a new 1RS.1BL translocation. This translocation has the long arm from the original 1RS.1BL translocation of ‘Kavkaz’, but a different 1RS arm. The third generation centric translocations were mitotically stable and were normally transmitted to progeny. Misdivision frequency of the reconstructed chromosomes 1R did not change relative to normal 1R, whereas the misdivision frequency of the two reconstructed chromosomes 1B tested was significantly higher relative to normal 1B. These experiments demonstrate that repeated cycles of centric breakage and fusion do not impair the function of centromeres in wheat and rye but may change chromosome's susceptibility to misdivision. This revised version was published online in July 2006 with corrections to the Cover Date.     

游离棉纤维长度的计算机测量

文中介绍了一种棉纤维长度测量的新方法,即用CCD捕获游离棉纤维图像,然后依据数字图像处理及模式识别的理论,建立起一个实际可行的算法,来完成对棉纤维多种长度指标的一次性测量。该算法涉及到数字图像的二值分割、补断、修剪、像素跟踪等几个方面,且针对棉纤维图像的特征,进行了优化。     

Descending Dimension Algorithm of Nolinear Programming Problem with Linear Inequality Constraints

The general nonlinear programming with linear constraints was investigated. In the algorithm, the method of contributing set was used. Comparing with the general contributing set method, the descending dimension method was used to solve the sub-problem with equality constraints in iterative procedure and the problem with inequality constraints. The algorithm is effective by the numerical test. Finally, it was proved that the iteration points are descending, when the objective functions are convex.     

考虑陀螺效应的动力总成悬置系统分析和优化

应用陀螺力学理论，对传统动力总成悬置系统的无阻尼动力学方程进行修正，引入陀螺矩阵，得到新的动力学方程。对新的动力总成悬置系统动力学模型进行计算分析，研究了陀螺矩阵对频域、时域响应的影响。使用遗传算法分别对修改前后的方程进行优化计算，并进行比较分析。结果表明，对于低转速发动机，曲轴转动的陀螺效应不明显，而对于高转速的发动机，陀螺效应不能忽略，应以考虑陀螺力矩的方程为基础进行设计分析。     

基于改进A *算法的电动车能耗最优路径规划

提出一种基于改进A*算法的电动车能耗最优路径规划方法。根据车辆运行时的能耗,考虑能量损失与回收等因素,建立了运行能耗函数。设计了新的启发式能耗预估代价对A*算法进行改进,证明了所提出的启发式能耗预估代价满足可采纳性和一致性,确保改进的A*算法可获得能耗最优路径。针对电动车的里程焦虑问题,基于改进的A*算法,建立了根据车载电池的剩余电量、充电站位置、终点位置来寻找可达的能耗最小路径方法。仿真实验表明,提出的方法可以找到起点到终点的能耗最小路径,当车载电池能量不足时,可以找到经过充电站的可行最小能耗路径,减少里程焦虑,验证了所提方法的合理性和可行性。     

利用改进BP神经网络预测初产奶牛产奶量的方法

针对传统BP算法的缺陷，提出了一种采用L-M训练法的BP神经网络。在此基础上建立了基于改进BP神经网络非线性系统的奶牛305d产奶量预测模型，此模型可以提前215d预测初产奶牛305d产奶量，从而实现提早进行选择，加速奶牛育种工作进程。并通过具体的实验验证了改进BP神经网络预测模型的有效性。